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Figure 7


Fig. 7. BR-C knockdown impairs cocoon spinning and pupal differentiation in Chrysopa. (A-C) Normal final (third) instar larva (A) spins a cocoon (C), in which it ecdyses into a pupa (B). (D-F) Injection of CpBR-C dsRNA into larvae kills them as prepupae; some of these larvae are unable to initiate or complete spinning of their cocoons (D). Upon removal of the larval cuticle (E,F), an arrested animal reveals pupal pigmentation, the development of compound eyes (white arrows) and short wings (black arrows), whereas typical larval mouthparts (stylets; A, black arrowhead) and long setae (asterisk) are missing. In contrast to in pupae, antennae are twisted above mouthparts, with their end oriented toward the anterior in CpBR-C(RNAi) animals (compare white arrowheads in panels B,E and F). Scale bars: 1 mm.





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