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Fig. 3. Expression profiles of the br isoforms. (A)
Expression profile of the br isoforms during development of the sixth
instar and the seventh instar larvae as determined by RT-PCR. (B)
Expression profile of the br isoforms during the development of the
hydroprene-treated and the acetone-treated seventh instar larvae as determined
by RT-PCR. The hydroprene-treated larvae molt to a supernumerary instar, and
the duration of the seventh instar is truncated relative to the
acetone-treated final instar larvae. Expression of rps3 is provided
to verify equal loading. Cycle numbers for br-Z1, br-Z2, br-Z3, br-Z4,
br-Z5 and rps3 are 38, 40, 34, 32, 35 and 37, respectively. D,
day.