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Fig. 4. Phenotypes obtained from larvae injected with br core
dsRNA. (A) Ventral and dorsal views of the whole body of the larva,
and the br dsRNA- and water-injected controls. Also shown are ventral
and dorsal views of a br dsRNA-injected supernumerary individual, the
old cuticle of which was removed. (B) Diagram of the body parts of
Tribolium discussed in the text (shaded in gray). (C)
Comparisons of the abdominal wall at the lateral side showing the presence and
absence of gin traps in the untreated larva, and in the br dsRNA- and
water-injected control animals. (D-I) Comparisons of the external
morphology of appendages in larvae, pupae (water-injected), br
dsRNA-injected individuals and adults. (D) The urogomphi of br
dsRNA-injected individuals tended to appear more larva-like. (E,F) Antennae
(E) and hind legs (F) of br dsRNA-injected individuals look more
adult-like, with distinct segments and forked claws at the end of the legs.
(G) Magnified view of the tarsus of the hind leg. Arrowheads indicate claws.
The pigmentation of the tarsal cuticle in the br dsRNA-treated
animals is more like that seen in the larvae. (H) The maxilla of br
dsRNA-treated animals has an adult-like morphology with larger palps (p), and
a more defined galea (ga) and lacinia (ln), but it retains the larval
cuticular pigmentation pattern. (I) The morphology of the mandible of the
br dsRNA-treated animal is most like that of the adult.
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