|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Phenotypes obtained following injection of different br isoform
dsRNA. (A) Water-injected control pupa. (B) Phenotype
obtained from knockdown of the br core region. (C) Phenotype
obtained from knockdown of all of the br isoforms. A close up of the
dorsal abdomen is shown with the reduced gin traps indicated by arrowheads.
(D-F) Phenotypes obtained from isoform-specific knockdown of
br-Z2 (D), br-Z3 (E), and a combination of br-Z2
and br-Z3 (F). Arrowheads indicate the tips of the reduced wings.
(G,H) Phenotypes obtained from isoform-specific elimination of
br-Z4 (G) and br-Z1 (H). (I) Pupa injected with
br-Z5 dsRNA looks normal. (J) Effect of br-Z2 and
br-Z3 dsRNA injection on the wings. Gray line marks the position of
the tip of the wings in the control animal. The double arrows indicate the
length of wings on each pupa. (K) Effect of isoform-specific knockdown
on the pupal tibia. Arrowheads point to the claws of the pupae; arrows
indicate the slightly augmented demarcation of claw region in br-Z1
and br-Z4 pupae. (L) Gin traps are reduced in pupae given
br-Z4 dsRNA. Double arrows show the length between the two gin trap
projections. (M) Normal adult. (N) Adult given br-Z2
dsRNA. (O) Adult given br-Z4 dsRNA. (P) Close-up of the
pupal-like cuticle on the dorsal thorax (arrowhead) and ventral abdomen
(arrow) in br-Z1 and br-Z4 dsRNA-treated animals. (Q)
A close-up of gin trap-like projections (arrow) seen on the dorsal adult
abdomen in br-Z4 dsRNA-treated adults.
|
