DEV007047 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Vimentin expression in the tongue does not change with Zeb1 mutation. (A,B) Vimentin immunostaining in the tongue of wild-type and Zeb1-null mice. (C,D) Co-localization of vimentin (C) and Zeb1 (D) in the tongue. E16.5.

• Supplemental Figure S2 -

  Fig. S2. E-cadherin expression expands to palatal mesenchyme with ZEB1 mutation. (A,B) E-cadherin expression remains on the tongue epithelium with Zeb1 mutation, but it does not expand to mesenchyme in the tongue. N, forming nasal cartilage; P, palatal mesenchyme; T, tongue. E16.5.

• Supplemental Figure S3 -

  Fig. S3. Mutation of Zeb1 leads to ectopic expression of E-cadherin and loss of vimentin expression in the olfactory lobe. (A,B) E-cadherin immunostaining. (C) Zeb1. (D-F). Vimentin. Arrows in C and F indicate similar locations. (F) A higher magnification view of the boxed region in E.

• Supplemental Figure S4 -

  Fig. S4. Immunostaining for BrdU. (A,D) Representative low-magnification views of embryos at E15.5 immunostained for BrdU. (B,C,E,F) Higher magnification of the boxed regions from A and D. Boxed regions in B and E are representative regions counted for quantification of BrdU incorporation in Fig. 4M. LLV, left lateral ventricle; RLV, right lateral ventricle; H, hypothalamus; T, tongue.

• Supplemental Figure S5 -

  Fig. S5. p15Ink4b expression. (A,B) p15Ink4b expression is similar in the skin of wild-type and Zeb1-null mice. However, expression is evident within and surrounding developing cartilage in the null mice. C, cartilage; S, skin.

• Supplemental Figure S6 -

  Fig. S6. Western blot analysis of 40 µg of protein from MEFs. 15% SDS gel. Antibodies were diluted 1:1000.