DEV012377 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. KBP regulates the speed of axonal outgrowth in the CNS. (A-A′′) Lateral views from a time-lapse series of the ventral spinal cord of a wild-type embryo with axons labeled by the Tg(HuC:kaede) transgene at 36 (A), 38 (A′) and 40 hpf (A′′). Anterior is towards the left and dorsal is towards the top. The arrowheads and red dots indicate the posterior-most axons. (B-B′′) Lateral views from a time-lapse series of the ventral spinal cord of a kbp^st23 mutant embryo with axons labeled by the Tg(HuC:kaede) transgene at 36 (B), 38 (B′) and 40 hpf (B′′). Anterior is towards the left and dorsal is towards the top. The arrowheads and blue dots indicate the posteriormost axons.

• Movie 1 -

  Movie 1. Time-lapse series of a wild-type Tg(HuC:kaede) embryo. Lateral view, with anterior towards the left and dorsal towards the top. Axons of the PLLn and ventral spinal cord migrate from anterior to posterior. The movie runs from 35-40 hpf, with 5-minute intervals.

• Movie 2 -

  Movie 2. Time-lapse series of a kbp^st23 mutant Tg(HuC:kaede) embryo. Lateral view, with anterior towards the left and dorsal towards the top. Axons of the PLLn and ventral spinal cord migrate from anterior to posterior. The movie runs from 35-40 hpf, with 5-minute intervals.

• Movie 3 -

  Movie 3. Time-lapse series of a kbp^st23 mutant Tg(HuC:kaede) embryo. Lateral view, with anterior towards the left and dorsal towards the top. Axons of the PLLn migrate from anterior to posterior. The movie runs from 54-60 hpf, with 5-minute intervals. Axonal behaviour is unstable and punctate motile axonal swellings appear that move backwards along the axon.