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Figure 3


Fig. 3. KBP is required for axonal development. Lateral views of PLLn axons in live embryos bearing the Tg(HuC:kaede) transgene at 56 hpf. The images show the segment of the nerve corresponding to about somites 10-16. Axons of the wild type (A) have advanced further posterior than those in the kbpst23 mutant (B). Axonal outgrowth is rescued in kbpst23 mutants injected with full-length wild-type kbp mRNA (C) and the mut2 kbp mRNA (E), but not in kbpst23 mutants injected with the mut1 kbp mRNA (D). Wild-type embryos injected with 2.4 ng of kbp morpholino (F) resemble kbpst23 mutants. In all images, anterior is towards the left and dorsal is towards the top.





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