|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Expression pattern of en-RFP-Moesin and ptc-GFP-Moesin
in the dorsal epithelium. (A) Schematic illustrating construction
of the fly line, transgene expression pattern and dorsal trichome pattern.
(B,C,F) Images of embryos expressing en-RFP-Moesin (red) and
ptc-GFP-Moesin (green). (Bi-iii) Dorsal view of embryos at the
start (i), midway through (ii), and shortly after completion (iii) of DC.
(Ci-iii) Time course of the latter stages of DC in a live embryo.
Misplaced ptc-GFP-Moesin-expressing cells (asterisks in i) are
present at a conserved position at the leading edge of the en domains
of all segments shown. (D) Bar chart showing proportion of embryos with
misplaced ptc-GFP-Moesin-expressing cells in each segment. (E)
Dorsal view of stage 16 embryo expressing GFP-Moesin (green) constitutively
and RFP-Moesin (red) under control of en-Gal4. Pairs of cells in the
P compartment not expressing RFP-Moesin are indicated by arrowheads.
(Fi-iv) Images from Movie 1 (see Movie 1 in the supplementary material)
showing cell rearrangements during DC that lead to the presence of the
misplaced ptc cell (asterisk) in the en domain. Scale bars:
10 µm.
|
