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Fig. 2. brk is required for PD axis specification. (A) A
brk- clone originating in the ventral proximal femur
marked by yellow and forked (arrow, outlined in white)
creating a cell-autonomous outgrowth. (A') The entire outgrowth
appears to have a single, tibia-like DV identity. co, coxa; tro, trochanter;
fem, femur; tibia, tib; tib', tibia-like outgrowth; t, tarsal segments 1-5;
cl, tarsal claw. (B) brk- clone proximal to the Dll
and Dac domains (absence of GFP, arrow) showing cell-autonomous derepression
of Dll and dac. No effects on Dll or dac
are observed in brk- clones within their normal domains
(asterisks). (C-D''') gro- (C) or
CtBP- (D) clones (absence of GFP, green) derepress
Dll and dac in proximoventral regions of the disc (arrows
and clones labeled `2'; isolated clones are outlined in white and shown in
C'', C''', D'' and D'''). No derepression is observed in
dorsal gro- or CtBP- clones
(arrowheads and clones labeled `1'; isolated clones are outlined in yellow and
shown in C' and D'). Clones within the Dac or Dll domains
(outlined, but not numbered) have no effect on Dac or Dll expression.
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