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Fig. 6. Oligodendroglial proliferation and survival in
Sox9loxP/loxP, Sox10lacZ/lacZ, Sox10::Cre
spinal cords. (A) Oligodendroglial proliferation was measured at
15.5 dpc and 18.5 dpc as the percentage of Sox10-positive cells (in the wild
type, white bars) or β-galactosidase-positive cells (in
Sox9loxP/loxP, Sox10lacZ/lacZ, Sox10::Cre
embryos, black bars) that also expressed Ki67. (B) The fraction of
Ki67-positive cells also labelled by a 1 hour pulse of BrdU was determined in
the wild-type and in Sox9loxP/loxP, Sox10lacZ/lacZ,
Sox10::Cre embryos as a measure of mitotic rates and cell cycle length.
(C) The Ki67-negative fraction of cells labelled by a single BrdU pulse
24 hours before embryo preparation was determined in the wild-type and in
Sox9loxP/loxP, Sox10lacZ/lacZ,
Sox10::Cre embryos as a measure of cell cycle exit rates. (D-G)
TUNEL was performed on transverse sections from the forelimb region of
wild-type (D,F) and Sox9loxP/loxP, Sox10lacZ/lacZ,
Sox10::Cre (E,G) embryos at 15.5 dpc (D,E) and 18.5 dpc (F,G). (H)
Apoptosis was quantified as the number of TUNEL-positive cells in wild-type
(white bars) and Sox9loxP/loxP, Sox10lacZ/lacZ,
Sox10::Cre (black bars) spinal cords at 15.5 dpc and 18.5 dpc. (I)
Apoptosis in OLPs was determined at 15.5 dpc and 18.5 dpc as the number of
TUNEL-positive, β-galactosidase-labelled cells in
Sox9loxP/loxP, Sox10lacZ/lacZ, Sox10::Cre
spinal cords (black bars) relative to the number in the
Sox10lacZ/+ spinal cords (grey bars) which was arbitrarily
set to 1. For all quantifications, at least 30 separate 10 µm sections from
the forelimb region of two independent embryos were counted for each genotype.
Data are presented as mean±s.e.m. Differences were statistically
significant for apoptosis rates in Sox9loxP/loxP,
Sox10lacZ/lacZ, Sox10::Cre spinal cords, as determined by
Student's t-test (***P
0.001).
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