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Fig. 2. Expression of Isl1 protein in the AHF and in myogenic progenitors of the
branchial arches. Immunofluorescence staining for Isl1 at different stages
of chick embryonic development [as illustrated in the top row, modified from
The Atlas of Chick Development (Bellairs
and Osmond, 1998)]. (A-C) Isl1 is expressed in the SpM and
the pharyngeal endoderm at St. 8. (D-F) At St. 12, Isl1 is restricted
to the ventral pharynx and the AHF/SpM at different anterior-posterior levels.
Myocardial cells in the linear heart tube show no Isl1 expression.
(G-I) Isl1 expression (St. 18) is detected in the mesodermal core of
BA1-2 but not in the neural crest-derived mesenchyme (nc). Isl1 is also
expressed in the aortic sac (as, H). (A'-I') Higher
magnifications of the regions indicated by the dashed squares in A-I. Arrows
in E' show Isl1 expression in the border of the SpM. Arrows in G'
and I' the expression of Isl1 in the mesoderm of BA1 and BA2,
respectively. nt, neural tube; ph, pharynx; ecto, ectoderm; endo, endoderm;
ba, branchial arch; SpM, splanchnic mesoderm; hrt, heart; oft, outflow tract;
ov, otic vesicle; CPM, cranial paraxial mesoderm; mes, mesoderm.
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