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Fig. 2. Both PSE1 and PSE2 contribute to PRE activity. (A) Construct
P[en2] contains 8 kb of upstream en sequences with PSE1 and PSE2
flanked by LoxP (L) and FRT (F) sites, respectively. (B-I)
Immunoperoxidase staining detecting β-gal protein in embryos from a
P[en2] line and its derivatives obtained by treating with Flp and/or Cre
recombinase. Embryos shown are 6 hours AEL (B-E) and 10 hours AEL (F-I);
anterior is leftwards, dorsal is upwards. All are lateral views, except C,
which is a ventrolateral view. Expression is initiated correctly in all lines,
then β-gal is misexpressed in cells between the stripes in 
PSE2,
PSE1 and both lines. Deletion of PSE2 causes relatively minor
misexpression (G). Deletion of PSE1 is more extensive (H), and deletion of
both causes the most misexpression between the stripes (I).
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