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Fig. 4. P[en3] inserted in tou is expressed in an en-like
manner. (A) The P[en3] construct contains en DNA from
-2407 bp to +188 bp. PSE1 is flanked by LoxP sites (L), PSE2 is flanked by FRT
sites (F). (B) Thick line indicates genomic DNA. Arrows below the line
indicate the transcription units and the direction of transcription of
en and tou. The start of tou transcription is 10 kb
upstream of what is shown. Arrows above the line indicate the position of
insertion of P[en3] in two independent lines, P[en3]-en and P[en3]-tou.
(C,D) Immunoperoxidase staining to detect β-gal protein in
embryos and X-gal staining to detect β-gal activity in wing discs of
lines P[en3]-en (C) and P[en3]-tou (D). Embryos are oriented with anterior
leftwards and dorsal upwards. Upper embryos are 
6 hours AEL. Lower
embryos are 10 hours AEL. β-Gal expression in stripes is not
initiated until about 6 hours AEL in P[en3]-tou embryos, and staining is
apparent within individual cells of stripes before the entire stripe is
stained. Wing discs were stained for equivalent amounts of time.
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