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Fig. 5. Effect of RA, PITX2 and Ad4BP/SF-1 on cyclin D1
expression.(A-H) Beads soaked in RA (B), RAA (C) and DMSO (D), and
chick embryonic fibroblasts expressing Pitx2 (E), Pitx2-en
(F), Ad4BP/SF-1 (G) and AP (H) were implanted into embryos
of both sexes as described in the legends to Figs
2 and
3. An untreated embryo is shown
in A. Embryos were fixed at stage 27, and cyclin D1 expression was examined by
whole-mount in situ hybridization. Sections of female (ZW) samples are shown.
Arrowheads (B,C,E-G) indicate altered expression of cyclin D1. Scale bars: 100
µm.
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