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Fig. 6. Effects of RA signaling and Ad4BP/SF-1 on the expression
ER
and CYP26A1. (A-K) Beads soaked
in RA (B,G), RAA (C,H) and DMSO (D,I), and chick embryonic fibroblasts
expressing Ad4BP/SF-1 (E,J) and AP (F,K) were implanted into
embryos of both sexes as described in Figs
2 and
3. An untreated embryo is shown
in A. The embryos were fixed at stage 27, and the expression of
ER
(A-F) and CYP26A1 (G-K) was examined by
whole-mount in situ hybridization. Sections of female (ZW) samples are shown.
Arrowheads in B and C, and in G and H, indicate affected expression of
ER
and CYP26A1, respectively. Scale bars: 100
µm.