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Fig. 3. PLK-1 cytoplasmic asymmetry is regulated downstream of MEX-5/6 and
upstream of PIE-1 in C. elegans. Micrographs of
two- to four-cell stage mutant or RNAi embryos (indicated at top).
(A-J) Merged immunofluorescence of anti-PLK-1 (green) and DAPI staining
(red). (K-M) GFP fluorescence of GFP::PLK-1PBD. (N-P)
Centrosomal and chromosomal anti-PLK-1 staining in one-cell wild-type (N) and
par-2(lw32) (P) embryos. A higher magnification photo of
wild-type mitotic chromosomes is shown in O. (Q) Centrosomal and
chromosomal GFP::PLK-1PBD signal in one-cell
mex-5(zu199);mex-6(RNAi) embryos.
(R) Quantification of PLK-1 asymmetry. The ratio represents the
relative intensity of anterior and posterior portions of one-cell embryos, or
somatic versus germline blastomeres in later stage embryos. Numbers in
parentheses are standard deviations. n, number of embryos measured.
Scale bar: 10 µm in all, except 2.5 µm in O.
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