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Figure 7


Fig. 7. PLK-1 and PLK-2 are required for MEX-5 and MEX-6 function in C. elegans. (A-L) Immunofluorescence micrographs of anti-MEX-5 and anti-GFP staining and corresponding DAPI staining in one-cell embryos expressing GFP::PIE-1. (A-D) Wild type (no RNAi). (E-H) plk-1(RNAi). (I-L) plk-1(RNAi);plk-2(RNAi). The arrows and arrowheads point to the oocyte- and sperm-derived pronuclei, respectively. Because of defective meiosis, the oocyte pronucleus has a higher DNA content than the sperm pronucleus in plk-1-depleted embryos. (M,O) DIC and (N,P) fluorescence micrographs of GFP::PIE-1 in four-cell wild-type (M,N) or mild plk-1(RNAi); plk-2(RNAi) (O,P) embryos. Closed and open arrowheads indicate the germline blastomere and its somatic sister, respectively. (Q) Quantification of MEX-5 and PIE-1 cytoplasmic asymmetry in one-cell wild-type, plk-1(RNAi), and plk-1(RNAi);plk-2(RNAi) embryos. See Fig. 3R legend for details. Scale bar: 10 µm. *, polar bodies.





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