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Fig. 2. svp mutant R1s and R6s express R8 or R7 rhodopsins with approximately equal likelihood. Adult retinas in which individual homozygous R1s and R6s created with GMR-FLP were labeled with mCD8-GFP (green) using MARCM. Rhabdomeres are visualized with phalloidin (red). Images are from the distal region of the retina, which contains the R1-R7 (identified in A) but not R8 rhabdomeres. (A-D) Retinas were labeled with antibodies against the two R8-specific rhodopsins Rh5 and Rh6 (both in blue). (A) Wild-type (FRT82) R1s and (B) R6s do not express R8 rhodopsins. Some svp mutant R1s (C) and R6s (D) express R8 rhodopsins. (E) Quantification of the experiment sampled in C and D. Blue bars represent mutant cells that adopted three R8 characteristics: a small, central rhabdomere, expression of Rh5 or Rh6 and a proximal nucleus. Green bars represent mutant cells that adopted three R7 characteristics: a small, central rhabdomere, a failure to express Rh5 or Rh6, and a distal nucleus. Gray bars represent mutant cells that retained R1/R6 characteristics. (F-I) Retinas were labeled with antibodies against the two R7-specific rhodopsins, Rh3 and Rh4 (both in blue). (F) Wild-type (FRT82) R1s and (G) R6s do not express R7 rhodopsins. Some svp mutant R1s (H) and R6s (I) express R7 rhodopsins. (J) Quantification of the experiment sample in H and I. Green bars represent mutant cells that adopted three R8 characteristics: a small central rhabdomere, a failure to express Rh3 or Rh4 and a proximal nucleus. Red bars represent mutant cells that adopted three R7 characteristics: a small central rhabdomere, expression of Rh3 or Rh4 and a distal nucleus. Gray bars represent mutant cells that retained R1/R6 characteristics. Scale bar: 5 µm.

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