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Figure 3


Fig. 3. Rhodopsin expression and target selection are correlated in svp mutant R1/R6s. Adult medullas in which the axon terminals of individual homozygous R1s, R6s and R7s created with GMR-FLP were labeled with synaptotagmin-GFP (green) using MARCM. All R axons were labeled with mAb24B10 (red). The approximate positions of the R8 recipient layer, M3, and the R7 recipient layer, M6, are indicated by broken lines. (A-D) Animals contained a transgene expressing lacZ under the control of the Rh6 promoter (Rh6-lacZ; blue). (A) Wild-type (FRT82) R7s never express Rh6-lacZ (arrows), whereas ~70% of wild-type R8s do (asterisks). (B) Approximately 70% of svp mutant R1/R6 axons that terminate in the R8 recipient layer express Rh6-lacZ (arrowhead), whereas most svp mutant R1/R6 and R7 axons that terminate in the R7 recipient layer do not (arrows). (C,D) We used the sev mutation to remove R7s. (C) Wild-type R8s all express Rh6-lacZ when R7s are absent. (D) svp mutant R1/R6 axons that terminate in the R7 recipient layer do not express Rh6-lacZ (arrow), while those that terminate in the R8 recipient layer do (arrowheads). (E-H) Animals contained a transgene expressing lacZ under the control of the Rh4 promoter (Rh4-lacZ; blue). (E) Approximately 70% of wild-type (FRT82) R7s express Rh4-lacZ (arrow; asterisks indicate heterozygous R7s expressing Rh4-lacZ); those that do not (double arrow) presumably express Rh3. (F) Approximately 70% of svp mutant R1/R6 and R7 axons that terminate in the R7 recipient layer express Rh4-lacZ (arrows), whereas most svp mutant R1/R6 axons that terminate in the R8 recipient layer do not (arrowhead). (G,H) We used the sev mutation to remove R7s. (G) When R7s are removed in wild type, there is no Rh4-lacZ expression. (H) Approximately 70% of svp mutant R1/R6s that terminate in the R7 recipient layer express Rh4-lacZ (arrow), while those that terminate in the R8 recipient layer do not (arrowheads). (I) Quantification of the experiments sampled in B and F, as well as of analogous experiments using Rh5-lacZ and Rh3-lacZ. Rh-lacZ expression was quantified in axons terminating in the R7 or R8 target layers of the medulla; each Rh-lacZ was examined in a separate experiment. Scale bar: 10 µm.





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