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Fig. 4. svp mutant R3s and R4s can become either R7s or R8s. Adult retinas in which individual homozygous cells created with ey^3.5-FLP were labeled with mCD8-GFP (green) using MARCM. The R1-R6-specific rhodopsin Rh1 was visualized with antibodies (red). Images are all from the distal region of the retina, which contains the R1-R7 (labeled in A and E) but not R8 rhabdomeres. (A-C) Retinas were labeled with antibodies against the two R8-specific rhodopsins, Rh5 and Rh6 (both in blue). (A) Wild-type (FRT82) R3s (arrow) and R4s (arrowhead) have large outer rhabdomeres that express Rh1 but not R8 Rhs. (B) Most svp mutant R4s (arrowhead) have small central rhabdomeres that no longer express Rh1, of which approximately half gain expression of R8 rhodopsins. (C) Most svp mutant R3s (arrow) retain large outer rhabdomeres that express Rh1 but occupy R4-like positions in ommatidia with reversed chirality. (D) Quantification of the experiment sampled in B and C. Blue and green bars represent mutant cells that adopted R8 or R7 characteristics, respectively (see Fig. 2E; in addition these lost Rh1 expression). Gray and black bars represent mutant cells that retained large outer rhabdomeres expressing Rh1 in ommatidia with normal or reversed chirality, respectively. (E-G) Retinas were labeled with antibodies against the two R7-specific rhodopsins, Rh3 and Rh4 (both in blue). (E) R7s but not wild-type R3s (arrow) or R4s (arrowhead) express R7 rhodopsins. (F) Approximately half of svp mutant R4s with small central rhabdomeres (arrowhead) express R7 rhodopsins (quantified in H). svp mutant R1/R6s generated by ey^3.5-FLP resemble those generated by GMR-FLP (double arrow indicates an R1). (G) As in C, most svp mutant R3s (arrow) retain large, outer rhabdomeres that express Rh1 but occupy R4-like positions in ommatidia with reversed chirality (quantified in H). (H) Quantification of the experiment sampled in F and G. Green and red bars represent mutant cells that adopted R8 or R7 characteristics, respectively (see Fig. 2J; in addition these lost Rh1 expression). Gray and black bars are as in D. Scale bar: 5 µm.

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