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Fig. 5. svp mutant R neurons co-express R7 and R8 markers during larval but not pupal development. Eye discs from wandering third instar larvae (L3; A-D'') or pupae 25 hours after puparium formation (P25; E-F''). ey-FLP^3.5 and the EGUF/hid method (Stowers and Schwarz, 1999) were used to create eyes homozygous for a wild-type (FRT82; A,C,E) or svp mutant (B-B'',D-D'',F-F'') chromosome. R cell nuclei were visualized with anti-Elav antibodies (red). Images are all from the apical region of the disc, which contains the R7 nuclei; R8 nuclei are not visible, except as noted in B. A proportion of R1/R6 and R3/R4 nuclei are visible. (A-B'') L3 eye discs were labeled with antibodies against the R7 and R8 marker Sal (green) and the R8 marker Sens (blue). Images are from row 20. (A) In wild-type L3 eye discs, R7s express Sal but not Sens, and R1/R6s and R3/R4s express neither. R8s (not visible) express both. (B-B'') In svp mutant L3 eye discs, most R1/R6s (solid circles) express Sal; some co-express Sens. R7s (asterisks) express Sal but not Sens, and R8s (the single visible R8 is marked with a carat) express Sal and Sens, as in the wild type. R3/R4/MCs (dashed circles) can also express either both Sal and Sens or Sal alone. (C-D'') L3 eye discs were labeled with antibodies against the R7 marker Pros (green) and the R8 marker Sens (blue). Images are from row 20. (C) In wild-type L3 eye discs, R7s express Pros but not Sens, R1/R6s and R3/R4s express neither, and R8s (not visible) express Sens but not Pros. (D-D'') In svp mutant L3 eye discs, most R1/R6s (solid circles) express Pros or Sens, and some express both (arrowhead). R7s (asterisks) express Pros only, and R8s express Sens only (not shown). R3/R4/MCs (dashed circles) can also co-express Pros and Sens (arrows). (E-F'') P25 eye discs were labeled with antibodies against Pros (green) and Sens (blue). (E) In wild-type P25 eye discs, R7s express Pros, and R8s (not shown) express Sens. (F-F'') In svp mutant P25 eye discs, svp mutant R neurons express either Pros or Sens but only rarely both (arrow). The disorganization of the svp disc at this stage prevents unambiguous identification of R1/R6 versus R3/R4 versus R7 neurons, but each ommatidium contains extra Pros or Sens-expressing R cells (see text). Scale bar: 5 µm.

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