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Fig. 5. Wnt-β-catenin signaling synergizes with that of
Ppar
to confer blastocyst competency for implantation.
(A,B) Overexpressing levels of Dkk1 (A) or PKF115-584 (B)
blocked activation of dormant blastocysts for implantation in response to
E2 (3 ng/mouse). Numbers within the bar indicate the number of mice
with implantation sites (IS)/total number of mice examined.
(C,D) Representative photomicrographs of uteri without blue
bands (C, right) and morphologically dormant blastocysts (D) recovered from
mice treated with PKF115-584. (E,F) Recombinant Wnt3a protein
(200 ng/ml) induced nuclear stabilization of active dephosphorylated
β-catenin and Ppar expression in dormant blastocysts in culture.
Co-treatment of Wnt3a with Dkk1 (1 µg/ml) or PKF115-584 (1 µM)
antagonized Wnt3a-induced β-catenin stabilization. Cy3-labeled antigens
in red, SYTO-13-labeled nuclei in green, and merge in yellow.
(G,H) Wnt3a and/or GW501516 conferred blastocyst implantation
competency. Dormant blastocysts were cultured in the presence of vehicle,
Wnt3a (200 ng/ml) and/or GW501516 (a selective Ppar agonist, 1 µM)
for 24 hours before transfer into pseudopregnant delayed recipients. Numbers
within the bar in G indicate the number of recipients with IS/total number of
mice examined, and those in H indicate the number of IS/total number of
blastocysts transferred; *P<0.05, Student's
t-test. Scale bars: 50 µm.
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