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Figure 2


Fig. 2. Expression of P-cadherin in the developing mouse hair follicle, eye and limb bud. (A) P-cadherin expression is upregulated in the HF placode at E15.5. (B) P-cadherin is expressed in the pigmented epithelium of the retina (white arrow) at E17.5. (C,D) Transverse sections of forelimb bud at E9.5 (C) and E10.5 (D) show prominent expression of P-cadherin along the AER (white arrows). Note that, in the transverse section, the tip of forelimb bud corresponds to the AER. For a better view, counterstaining with DAPI is shown in red instead of blue (B-D). (E,F) Longitudinal section of forelimb bud at E15.5 shows that P-cadherin is widely expressed throughout the epithelial cells of the forelimb bud. F is a higher magnification view of E. (G,H) Whole-mount immunohistochemistry also localizes the P-cadherin in the AER of the forelimb bud at E9.5 (white arrows). Scale bars: 100 µm.





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