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Figure 1


Fig. 1. RMCE in the mouse Ednra gene. (A) Strategy for targeting and knock-in of lacZ. Probes for genotyping are indicated as 5'- and 3'-probes. B, BamHI; Bl, BlnI; C, ClaI; P, PstI; Pm, PmeI. (B) Southern blot analysis of PstI- or BlnI/PmeI-digested genomic DNA of the offspring from an intercross of heterozygotes, probed with the 5'- or 3'-probes, respectively. (C) Genomic PCR analysis for sequential Cre-lox-mediated recombination with primers indicated in A.





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