DEV008631 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. DNA methylation at the RFT1 locus in Hd3a RNAi plants. (A) Schematic diagram of RFT1. Genomic DNA digested with McrBC was amplified by PCR using specific primers (regions MI-MIII). (B) DNA methylation analysis of RFT1 in wild-type plants and Hd3a RNAi plants 35 and 70 DAS (ZT 0) under SD conditions. NM, non-methylated (NM) genomic rice DNA (M65) and methylated (M) genomic rice DNA (35S-Pi) were used as controls (Okano et al., 2008).

• Supplemental Figure S1 -

  Fig. S2. Developmental expression analysis of RFT1 in Taichung65 (T65) under SD conditions. (A) Expression of Hd3a in T65, Norin8 and Hd3a RNAi plants. Expression of Hd3a was low at all developmental stages in T65, which contains loss-of-function Hd1 and Ehd1 alleles. (B) Expression of RFT1 in T65, Norin8 and Hd3a RNAi plants. RFT1 transcription was very low at all developmental stages in T65 (red line). Leaves were collected from T65, Norin8 and Hd3a RNAi plants at 35, 50, 70 and 90 DAS (ZT 0) under SD conditions.