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Figure 3


Fig. 3. Functional analysis of RFT1 by RNAi under SD conditions. (A) Hd3a expression in RFT1 RNAi, Hd3a RNAi and double RFT1-Hd3a RNAi plants under SD conditions. (B) RFT1 expression in RNAi plants under SD conditions. Leaves were sampled 35 DAS (ZT 0) under SD conditions. Expression of Hd3a and RFT1 was examined by real-time PCR. Black, wild-type; blue, RFT1 RNAi plants; red, Hd3a RNAi plants; green, double RFT1-Hd3a RNAi plants. (C) Flowering time of RNAi plants under SD conditions. (D) RNAi constructs. Gene-specific regions of Hd3a and RFT1 were used for the Hd3a and RFT1 RNAi constructs. 5'UTR of RFT1 and 3'UTR of Hd3a were used for the double RFT1-Hd3a constructs. (E) Growth of wild-type and RFT1-Hd3a RNAi plants. Ri 1-3, RFT1 RNAi plants; Hi 1-3, Hd3a RNAi plants; Di 1-2, double RFT1-Hd3a RNAi plants. Scale bars: 10 cm.





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