Role of epithelial cell fibroblast growth factor receptor substrate 2 in prostate development, regeneration and tumorigenesis

DEV009910 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Ablation of Frs2αalleles inhibits prostate growth. (A) Prostates were dissected from mice at the indicated ages, and individual lobes were dissected. Frs2α^cn prostates were smaller than the controls. (B) Prostate tissues from 6-week-old mice were sectioned and stained with Hematoxylin and Eosin (HE). Insets are high-magnification views of the same tissues. F/F, homozygous Frs2α^flox mice; CN, Frs2α^cn mice; a, anterior prostate; dl, dorsolateral prostate; v, ventral prostate.

• Supplemental Figure S2 -

  Fig. S2. Frs2α^cn prostates express markers of differentiation and secretory proteins. (A) Prostate sections from 8-week-old mice were immunostained with anti-α-actin, anti-cytokeratin 8 and anti-androgen receptor antibodies as indicated. (B) The PBS-extracted proteins from prostatic lobes of 8-week-old mice were separated by 5-20% gradient SDS-PAGE and stained with Coomassie Brilliant Blue G250. (C) The secretory proteins collected from the indicated lobe of 2-month-old mice were separated by SDS-PAGE and western blotted with anti-probasin or anti-PSP94 antibodies as indicated. F/F, homozygous Frs2α^flox mice; CN, Frs2α^cn mice; M, molecular-weight markers.

• Supplemental Figure S3 -

  Fig. S3. Frs2α^cn prostates remain androgen-dependent with respect to tissue homeostasis. Hematoxylin and Eosin (HE) staining of sections of prostate dissected from mice prior to the castration (Control), 2 weeks after castration (Castrated), and 2 weeks after restoration of androgen (Regenerated). CN, Frs2α^cn mice; F/F, Frs2α^flox homozygous mice.