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Figure 2


Fig. 2. Disruption of Frs2{alpha} alleles inhibits ductal branching morphogenesis in prostates. (A) The urogenital tract was dissected at the indicated times, lightly fixed and then stained with X-Gal. Stained tissues representing each prostatic lobe are shown. Note the lack of significant differences between Frs2{alpha}cn (below) and control (above) prostate rudiments at E17.5 or later. (B) Prostatic lobes were dissected from mice at the indicated ages. The tissues were lightly fixed and stained with X-Gal (a,b). Note that only epithelial cells in the prostate were stained. The ductal network in each prostatic lobe from 4-week-old mice was microdissected (c-e) and the average number of tips was quantified from three prostates and is shown as mean±s.d. (f). AP, anterior prostate; DLP, dorsolateral prostate; VP, ventral prostate; B, bladder; S, seminal vesicles; U, urethra; F/F, homozygous Frs2{alpha}flox mice; CN, Frs2{alpha}cn mice; WT, wild-type Frs2{alpha}.





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