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Fig. 3. meis1 is required for the G1-S transition and the expression of
the G1-S regulators cyclin D1 and c-myc. (A-D)
Histograms displaying DNA content (cell cycle profile) of cells from dissected
19hpf eyes of meis1-MO treated embryos relative to (A) controls
(Crtl-MO), (B) meis1-MO+GFP-meis1 mRNA, (C)
meis1-MO+cyclin D1 mRNA, and (D)
meis1-MO+c-myc mRNA injected embryos. Number of replicates
(n) and P values are indicated. meis1 knockdown
induces a delay in G1, which is partially rescued by co-injection of
GFP-meis1 (B), cyclin D1 (C) and c-myc (D) mRNAs.
(E) Average percentages are shown for G1, S and G2/M DNA content. The
cell-cycle profiles of control morphants and of uninjected, wild-type embryos
are indistinguishable (not shown). (F-I) In situ analysis of cyclin
D1 and c-myc transcription in control (F,H) and meis1
morphants (G,I) at 19hpf. meis1 morphants show a dramatic reduction
in cyclin D1 and c-myc in the eye (red arrowheads).
cyclin D1 and c-myc are still detected in other body regions
(black arrowheads). Hybridization and reaction development were performed
strictly in parallel. Representative embryos are shown.
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