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Figure 1


Fig. 1. Spatial and temporal expression of Meis1 and Meis2. (A-C,E) Expression of Meis2 in chick at (A) HH7 (arrow, anterior neural plate), (B) HH15 (E2; arrow, eye cup), (C) HH19 (early E3) and (E) HH25 (E4.5). Meis2 expression is lost in RPCs after late E2. Arrows in C indicate the central domain, where Meis2 is downregulated. (D) Expression of Ath5 in chick at HH19. Arrows indicate where Ath5+ cells appear at HH19. (F-F'') Double immunostaining for Meis2 and Pax6 at HH11. (G-J) Expression of Meis1 in chick at (G) HH11, (H) HH15, (I) HH19 and (J) HH25. Arrow in J indicates Meis1 downregulation in the RGC layer. (K) Expression of Meis2 in mouse eye anlage at E9.5. (L) Meis2 is not expressed in the E12.5 mouse retina. (M,N) Meis1 expression in the mouse optic vesicle (M) and neural retina (N). Expression is downregulated in the RGC layer (arrow). Boxed areas in E and J are shown at high magnification in the insets. Le, lens; lp, lens placode; mes, mesencephalon; nR, neural retina; ov, optic vesicle; PE, pigmented epithelium; SE, surface ectoderm. Scale bars: 200 µm in C,D,I; 500 µm in B,E,H,J; 50 µm in K,M; 100 µm in L,N.





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