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Fig. 2. Forced expression of a dominant-negative Meis interferes with RPC
proliferation. (A-C) MeisEnR-transfected chick eyes are
microphthalmic. (D-E') BrdU incorporation following ectopic
expression of GFP (D,D') or MeisEnR together with
GFP (E,E'). (F) Quantification of the results from
D-E'. Error bars indicate s.d. (G,H) TUNEL labeling of
HH16 retinae transfected with nuclear red fluorescent protein (nRFP;
G) or MeisEnR together with nRFP (H). (I) TUNEL
labeling on a HH16 chick retina treated with DNase I to visualize the
effectiveness of the labeling strategy. (J-L) Clonal analysis.
Representative clones infected with RIS(B)-GFP (J),
RIS(B)-Meis2 (K) and RIS(B)-MeisEnR (L) visualized with the
virus-specific antibody p27 (green). RIS(B)-MeisEnR-infected cells
express the Myc-tag C-terminally fused to EnR (red, L). (M)
Number of cells per clone and per section plotted as a function of the
frequency at which clones of the respective sizes were observed.
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