|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Decrease in Pax6, Six3 and Chx10 expression
following MeisEnR transfection. (A-H) Expression of
Pax6 (A-D), Chx10 (E,F) or Six3 (G,H) in HH15 chick
eyes transfected with control-EnR (A,C,E,G) or MeisEnR
(B,D,F,H). (A,B,E,F) Wholemounts. (C,D,G,H) Vibratome sections. (I)
Quantification of the gene expression changes observed. (J) RT-PCR of
two independent experiments (Exp_a, Exp_b) for Pax6 and β-actin
of chick HH15 eyes transfected with GFP (control) or M1si_I and
M2si_I together (Msi). PCR conditions were: 30 seconds 94°C, 30 seconds
60°C, 1 minute 72°C for 27 cycles. (K) Relative pixel intensity
of the ethidium bromide-stained bands from J (Pax6/β-actin).
Simultaneous knock-down of Meis1 and Meis2 expression
decreases Pax6 transcript levels in the eye. (L,M)
Pax6 fails to rescue cyclin D1 expression in
MeisEnR-transfected chick retinae. Arrows indicate domains of
MeisEnR/Pax6 misexpression and reduced cyclin D1 expression. (M)
cyclin D1 expression following transfection of MeisEnR together with
Pax6. (N-P) Expression of cyclin D1 (N) or cyclin D2 (P)
following Pax6EnR misexpression. (O,L) Transfection control. L,M and
N-P are neighboring sections. (Q) Quantification of the observed gene
expression changes from Fig.
3F-J and data not shown.
|
