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Fig. 3. Non-reciprocal genome-wide dependence of E(z) on Pcl for chromosome
binding. Polytene chromosomes from (A) Oregon R, (B)
E(z)61, or (C) pUAST-R57-Pcl/P{GawB}c729 larvae
were stained with anti-E(z) or anti-Pcl (red) and anti-IIoSer2
(green). (A) Wild-type distributions of E(z) and Pcl. (B) Inactivation of
E(z)61 does not affect chromosome binding by Pcl. (C) Pcl knockdown
results in loss of chromosome binding by E(z) (background debris signal is
indicated by an arrowhead).