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Figure 2


Fig. 2. Groucho is phosphorylated in cellular blastoderm embryos at the termini and in seven central transverse stripes. (A-C) Lateral view of a stage 5 blastoderm embryo, double-stained with {alpha}pGro (A; red) and {alpha}Gro (B; green) antibodies. (C) Merge. Phosphorylated Gro is detected at both poles, and in seven stripes in the trunk region of the embryo (A). {alpha}Gro staining is complementary to that of {alpha}pGro (C). (D) The pGro signal is significantly reduced in embryos devoid of maternally contributed Gro (germline clone; GLC), stained by {alpha}pGro antibodies. (E-G) Confocal optical cross-sections of a stage 4 syncytial blastoderm embryo, stained for {alpha}pGro (E; red) and {alpha}dpERK (F; green). (G) Merge. {alpha}dpERK and {alpha}pGro staining colocalises at the anterior and posterior termini. dpERK is mostly cytoplasmic, whereas pGro is predominantly nuclear. In this and subsequent figures, embryos are oriented with the anterior towards the left and dorsal side upwards.





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