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Figure 4


Fig. 4. Phosphorylation of Groucho and downregulation of its repressor function are required for terminal gap gene expression. Expression of tll (A-D) and hkb (E-H) in stage 4 embryos maternally expressing Gro (B,F), GroAA (C,G) or GroDD (D,H). Embryos similarly expressing lacZ serve as controls (A,E). Expression of Gro leads to reduction in tll and hkb transcription at both termini (B,F), whereas that of GroAA almost completely eliminates tll and hkb expression (C,G). In the posterior of the embryo, tll and hkb expression remains only in a ventral-terminal domain (arrows in B,C,F,G). Expression of GroDD has no effect, indicating that it cannot repress tll and hkb (D,H).





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