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Fig. 2. pxt is expressed throughout oogenesis and required for egg
maturation. (A) Schematic of the pxt gene, showing the
5' and 3'UTR as white boxes, the exons as gray boxes, the
alternative splice exon as a black box, the introns as lines and the
insertions as black triangles. (B) RT-PCR and qRT-PCR (numbers below)
showing pxt expression in whole ovaries from the indicated genotypes.
(C) Female fertility of pxt genotypes relative to wild type
2-5 days (dark gray) or >15 days (light gray) after eclosion. (D)
pxtf01000 egg chamber showing a nurse cell dumping defect
(asterisk marks nurse cells). (E-I) Whole-mount in situ hybridization
using a pxt probe. (E) pxt is expressed weakly early in
oogenesis, with an upregulation of expression beginning at S6. (F,G)
pxt is expressed in both the germ and somatic cells, including border
cell expression (F,F', arrows) and mainbody follicle cells (outlined in
G). (H,I) Expression remains strong throughout the rest of oogenesis. The
probe concentration is higher in E than in F-I. F' shows DAPI. Scale
bar: 50 µM.
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