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Figure 3


Fig. 3. pxt mutants are rescued by pxt cDNA, exogenous PGs and mouse Cox1. (A) Fertility of pxtf01000 mutant females (over a 4-day period) is rescued by expression of a UASp-pxt+ transgene driven by heat shock-GAL4 (HS GAL4) or the somatic ovarian driver c587. (B) Rescue of pxtf01000 developmental arrest in vitro by added PGH2 (10 µM) or fluprostenol (Flu) (20 nM). t-tests were performed comparing drug treated (blue) with drug plus prostaglandin (yellow or red). DIC image of a pxtf01000 stage 10B egg chamber developed in vitro without PG (C) showing residual nurse cells (*) or with added 60 µM PGH2 showing rescue (D). (E) Fertility of pxtf01000 mutant females (over 4 days) is rescued by expression of a UASp-MCOX1 transgene driven by heat shock-GAL4 (HS GAL4). (F,G) MCOX1 expression (G) rescues nurse cell dumping (arrows indicate nurse cell nuclei) and dorsal appendage (outlined) formation. Projections of confocal sections of S14s from HS GAL4; Sco/CyO; f01000 (F) and HS GAL4; UAS MCOX1/balancer; f01000 (G). Green is 1B1 (marking membrane) and blue is DAPI. Scale bar: 50 µM.





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