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Fig. 4. Loss of pxt and COX inhibitors disrupt actin bundles required
for nurse cell dumping. (A-F') Actin bundles in wild-type
nurse cells in cytoplasmic (A,A') and subcortical regions (D,D')
are greatly reduced in pxt-/- mutants (B,B' and
E,E', respectively), and restored by expression of MCOX1 under the
control of HS GAL4 (C,C' and F,F'). (G-I) Actin bundles in
stage 10B egg chambers developed in vitro until stage 11 in the absence (G) or
presence (H) of 1.5 mM aspirin, or with aspirin and 60 µM PGH2
(I). The presence of aspirin disrupts subcortical actin and causes actin
aggregation in dumping nurse cells (H), which is partially reversed by PG
addition (I). A-F are projections of several confocal sections.
A'-F' are zoomed images of one nurse cell from the adjacent image.
Red is phalloidin and blue is DAPI. (E-G) Epifluorescent images. Scale bars:
50 µM.
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