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Fig. 1. Embryonic expression of mib2 in Drosophila.
(A-D) mib2 RNA in situ hybridisations to wild-type embryos.
(A,B) In stage 11 embryos, mib2 is expressed in founders of the
somatic (arrowheads) and circular visceral (arrow) muscles. (C) Detail of a
late stage 12 embryo (inset). mib2 expression occurs in founders of
the longitudinal visceral muscles (arrow). (D) At stage 14, mib2
expression is maintained in muscle precursors and is also detected in
cardioblasts (asterisk). (E-G) Mib2 (green) accumulates in founders of
circular visceral muscles (E, arrow) and in somatic founders, revealed by
anti-β-gal accumulating in the nuclei (red) in rP298-lacZ
embryos (F, enlarged in G). (H-I'') Mib2 (green) accumulates in
the cytoplasm but not in Connectin-labelled membranes (red, arrows in
H-H''). Mib2 is not enriched at muscle attachments (anti-Kettin, red,
arrows in I-I''). See Fig.
3 for a diagram of the muscle pattern. In all embryos, anterior is
to the left and dorsal to the top, except for the ventral view in E.
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