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Fig. 4. Embryonic rescue of mib2 phenotypes and ultrastructure organisation of mib2 larval muscles. (A-C) Dorsal region of stage 17 kettin-GFP (A), mib2¹; kettin-GFP (B) and mib2¹; kettin-GFP rescued with UAS-mib2-C935S-C1020S (C) embryos. Note the alignment of Z bands in the DO1/2 muscles in A and C (arrowheads), and the distorted (arrow), irregular (asterisk) or absent (arrowhead) bands in B. Images in A and C also show the Z bands of the immediately overlying DA1/2 muscles (see F for dorsal muscle scheme). (D-E') Mesodermal phenotype of stage 16 mib2¹ embryos (anti-myosin stained), expressing UAS-mib2-FL (D,D') and UAS-mib2-C935S-C1020S (E,E') (24BGAL4 driver). Gut constrictions (D,E) and external (D',E', left) and internal (D',E', right) views of the ventral muscles schematised in G are shown. Mib2-FL completely rescues the mutant phenotype (D,D', compare with Fig. 3A,B). Mib2-C935S-C1020S rescues all embryonic phenotypes except the fusion defect, indicated by the presence of unfused myoblasts (arrowhead in E') and number of DA1 nuclei (Fig. 3F). Some embryos also failed to complete the first gut constriction (arrowhead, E), a phenotype common to most fusion mutants. (F) Scheme indicating dorsal muscles. (G) Scheme indicating ventral muscles (brown) missing in Fig. 3B,C.

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