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Figure 5


Fig. 5. The AKT downstream proteins and EG cell lines derived from p53-deficient mice. (A-C) Immunostaining with antibodies against phospho-GSK3 (A), MDM2 (B) and Ser20-phospho-p53 (C). The AKT-MER transgenic PGCs were cultured for 2 days in the presence of the indicated reagents. The cells were stained with antibodies against AKT downstream molecules (red) and germ cell markers SSEA-1 or PGC7/Stella (green). Yellow and white arrowheads indicate the stained and unstained PGCs, respectively. The percentage of positive cells is shown below each picture. The percentage of phospho-GSK3-positive PGCs (A) and of PGCs stained strongly with MDM2 antibody (B) was significantly higher in the 4OHT-treated PGCs, irrespective of bFGF treatment (*P<0.005, {chi}2 test), than in wild type. The percentage of phospho-p53-positive PGCs cultured without 4OHT and bFGF was significantly higher than that of other groups (*P<0.005, {chi}2 test; C). More than 300 PGCs were analyzed in each group. Scale bars: 25 µm. (D) EG cell lines derived from p53-deficient mice. EG cell lines were established in the presence (left) or absence (right) of bFGF. Scale bar: 100 µm.





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