DEV011569 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Phenotype of the cerebellar hemispheres in mes/r1-S2GOF;F8 animals. (A-H) Sagittal sections through the cerebellar hemispheres of control and mes/r1-S2GOF;F8 adults (P28) stained with Cresyl Violet or an antibody against calbindin. In more lateral sections (A-D), the hemispheres are somewhat smaller, whereas more medially (E-H), there is significant tissue loss in the mutant. The inferior colliculus is absent in the mutant. (E′-H′) The areas outlined in E-H are shown at higher magnification. The granule cell layer is of normal thickness and Purkinje cells are present and appear normal. Abbreviations: GCL, granule cell layer; IC, inferior colliculus; PCL, Purkinje cell layer; SC, superior colliculus; WM, white matter.

• Supplemental Figure S2 -

  Fig. S2. Gene expression near the isthmic organizer in mes/r1-S2GOF;F8 embryos. (A-F) Lateral and (A′-F′) dorsal views of control and mes/r1-S2GOF;F8 embryos at the somite stages indicated, assayed by whole mount in situ hybridization for expression of (A-B′) Otx2 and Hoxa2, (C-D′) Gbx2 and (E-F′) Wnt1. The red arrowhead in B′ points to cells in r1 that are ectopically expressing Otx2. The black arrows in D and F indicate the isthmic constriction. Gbx2 expression in r1 and Wnt1 expression in the posterior mes is reduced in the mutant embryos.