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Fig. 2. Expression of a single copy of the recombined Spry2-GOF allele
in mes/r1 causes absence of the inferior colliculus and anterior vermis.
(A,B,E-I) Midsagittal sections of brains of the genotypes
indicated, collected at the stages denoted and stained with Cresyl Violet or
Hematoxylin and Eosin (I). Anterior is towards the left, posterior is towards
the right. In control embryos, the posterior midbrain and anterior hindbrain
regions that are absent in the mutant embryos are demarcated by red and yellow
broken lines, respectively; in the mutant embryos, the regions where the
missing midbrain and anterior hindbrain tissue would normally be found are
indicated by red and yellow asterisks, respectively. (C,D)
Higher power view of the tissue shown in A and B, respectively, stained with
an antibody against calretinin (CR). The CR-negative inferior colliculus in
the control is indicated by a broken red line. In the mutant, this CR-negative
region, as well as the isthmus and velum medullare are absent (arrowhead).
(G-I) Comparison of vermis development in control, mes/r1-S2GOF, and
Fgf17-/-; Fgf8+/- animals at 
3
weeks of age. CP, choroid plexus; IC, inferior colliculus; SC, superior
colliculus; VM, velum medullare. The lobules of the vermis are indicated by
Roman numerals, as described by Inouye and Oda
(Inouye and Oda, 1980).
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