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Figure 4


Fig. 4. R/C patterning in Lfng{Delta}FCE1/{Delta}FCE1 embryos. (A) Whole-mount in situ hybridization for Mesp2, defining the presumptive rostral compartment of the pre-somite. In wild-type (a,c) and Lfng{Delta}FCE1/{Delta}FCE1 (b,d) embryos, a single clear band of Mesp2 expression is seen at both 9.0 (a,b) and 10.5 (c,d). The anterior border of this band is sometimes less defined in Lfng{Delta}FCE1/{Delta}FCE1 embryos (arrows). (B) Whole-mount in situ hybridization with a probe against Uncx4.1, which demarcates the caudal half of the somites. At 9.5 and 10.5 dpc, wild-type somites have clear rostral and caudal compartments (a-d). During primary body formation, Lfng{Delta}FCE1/{Delta}FCE1 embryos exhibit some compartmentalization with stronger staining in the caudal region of the somite (e,f, arrowheads), although compartments are frequently irregular (e, bracket). At this stage, little compartmentalization in seen in Lfng-/- embryos (i,j). The mature derivatives of these somites are patterned; clear rostral compartments are observed in Lfng{Delta}FCE1/{Delta}FCE1 embryos in the sclerotome of mature somites in the thoracic region, but compartments may be misshapen or irregularly spaced (arrow, g). Somites in the thoracic region of Lfng-/- embryos exhibit no compartmentalization at this stage (k). During secondary body formation, somites in Lfng{Delta}FCE1/{Delta}FCE1 embryos are of regular size and are correctly patterned (h), while in Lfng-/- embryos at this stage, little to no compartmentalization is observed (bar, l). (C) Whole-mount in situ analysis of Mox1 mRNA demonstrates a regular pattern of mature somitic derivatives in the thoracic region of wild-type embryos at 10.5 dpc. (a) In Lfng{Delta}FCE1/{Delta}FCE1 embryos, somitic derivatives in this region are distinct but irregularly spaced (b, arrow, bar). (D) Staining with 2H3 reveals the regular pattern of axon projections in the trunk region of wild-type embryos at 10.5 dpc (a). In Lfng{Delta}FCE1/{Delta}FCE1 embryos, these projections are spaced irregularly (b, arrow). Anterior is towards the left in all panels. *Hindlimb bud.





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