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Figure 7


Fig. 7. Oscillatory gene expression is variously perturbed in Lfng{Delta}FCE1/{Delta}FCE1 embryos. (A) Nrarp expression oscillates in wild-type embryos at 8.5 dpc (a,b, n=10), but is stably expressed in Lfng{Delta}FCE1/{Delta}FCE1 embryos (c,d, n=6). At 10.5 dpc, wild-type embryos exhibit two distinct patterns of expression (e, n=7; f, n=8). Both these phases are seen in Lfng{Delta}FCE1/{Delta}FCE1 embryos, but the pattern is more diffuse than that observed in wild-type embryos (g, n=5; h, n=2). (B) Cyclic expression of the Dll1 intron probe is observed in both wild-type (a, n=6; b, n=7) and Lfng{Delta}FCE1/{Delta}FCE1 (c, n=6; d, n=4) embryos at 10.5 dpc. (C) In wild-type embryos, two phases of Hesr1 expression are seen, with a narrow band in the anterior PSM and either a broad (a, n=4) or narrow (b, n=2) band in the posterior PSM. Hesr1 expression is perturbed in Lfng{Delta}FCE1/{Delta}FCE1 embryos, with a diffuse band of staining extending from the posterior into the anterior PSM that overlies a narrow band of expression in the anterior PSM (c, n=6).





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