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Fig. 3. Expression patterns of genes associated with early development in mouse
Rex1+/- populations. (A) Ratio of expression
levels of early genes in Rex1+ and
Rex1- populations were examined by Q-PCR. The averages of
values from RNA of Rex1+ and Rex1-
cells sorted three times independently are shown in the graph. (B)
Immunostaining of Rex1-EGFP/Oct3/4-ECFP double-knock-in cells with antibodies
for proteins expressing in the peri-implantation embryos. Fluorescence of
antibody reaction is shown as red signal (Alexa 594) for Nanog, Klf4 and Tbx3,
or purple signal (Alexa 647) for Esrrb and Foxd3.
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