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Figure 4


Fig. 4. Reversible phenotypes of mouse Rex1+ and Rex1- populations. (A) Rex1+/Oct3/4+ and Rex1-/Oct3/4+ fractions were sorted, cultured and analyzed by flow cytometry. Oct3/4-CFP/Rex1-GFP double-knock-in ES cells were cultured under puromycin selection, then Rex1+ and Rex1- populations were purified at more than 98% purity. Rex1- cells emerged from the purified Rex1+ population, and Rex1+ cells also appeared from purified Rex1- populations within 1-2 days. It was confirmed that GFP fluorescence reflected Rex1 expression by RT-PCR. (B) Clonal analysis of reversibility of Rex1+ and Rex1- cells. Scale bar: 50 µm.





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