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Fig. 7. Differentiation ability of
Rex1-/Oct3/4+ cells into mesodermal
and neural lineages. (A) Dot-plots of analysis at day 0 and day 4
for mesodermal induction from unselected Rex1-tk2 knock-in ES cells.
Percentage of Flk1+/E-cadherin- cells (indicted by
rectangles) was measured by analyzer. (B) The results of induction of
mesoderm from Rex1+/Oct3/4+ and
Rex1-/Oct3/4+ populations selected
with GANC and puromycin. Note that
Rex1-/Oct3/4+ (PrE-like cells)
differentiated into mesodermal cells more efficiently and with faster kinetics
than non-fractionated ES cells. (C) Analysis of induction of neural
cells (dot-plot on day 4). Anti-NCAM antibody and Alexa-633-conjugated
anti-goat IgG antibody were used as first and second antibodies, respectively,
for measurement of the proportion of neural cells. Green line, control ES
cells stained with only secondary antibody; blue line, fraction of
Oct3/4+ cells on day 5; red line, enriched
Rex1-/Oct3/4+ fraction on day 5.
(D) Summary of experiment for induction of neural cells on days 2, 4
and 5.
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