DEV010660 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Pharyngeal pax9 expression is not expanded in kgg mutants at 48hpf. (A,B) Ventral view. Magnification: 100×.

• Supplemental Figure S2 -

  Fig. S2. kgg phenotype persists into larval stages. The esophageal-intestinal junction, indicated by the black arrow, is shifted posteriorly in kgg larvae at 7dpf. The esophageal region, indicated by asterisks, may be expanded. (A) Wild type. 100×. (A′) Specimen from A shown at 200×. Note the distinct junction between the posterior pharyngeal epithelium, indicated by white arrowheads, and the pale-staining columnar esophageal epithelium. (B) kgg mutant. 100×. The notochord is partially in the plane of section of the somites because of medial-lateral curvature. (B′) Specimen from B shown at 200×. The epithelium posterior to the fifth gill arch is ambiguous in appearance and we could not identify a pharyngeal-esophageal junction. (C) Anterior endodermal epithelium of another kgg specimen, shown at 200×. Wild type, n=4; kgg, n=6. Numbers indicate somites. E, esophagus; IB, intestinal bulb; L, liver; N, notochord; SB, swim bladder; Y, yolk; black arrow, esophageal-intestinal junction; white arrow, fifth gill arch; black arrowhead, pancreas; white arrowhead, epithelium posterior to fifth gill arch. Hematoxylin and Eosin-stained sagittal sections, anterior to left.