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Fig. 4. Cdx4 has roles in determining β-cell localization, cell
number and in regulating β-cell convergence to the midline during
early pancreatogenesis. (A) Summary of
Fig. 3 data showing average
(modal) insulin domain locations for post-16hpf timepoints relative
to somite number (shown as numbered boxes). mut, kgg mutants
(cdx4-/-); sib, siblings of kgg mutants,
including both heterozygotic and wild-type (wt) zebrafish clutchmates.
(B) The number of β-cells increases more rapidly in
cdx4-/- embryos than in siblings (mean±s.d.).
(C) Midline convergence of β-cells is delayed in
cdx4-/- embryos. y-axis indicates percentage of
embryos in which β-cells have converged to the midline. (D)
Summary of Fig. 5 data showing
modal insulin and pdx1 domain locations for
Cdx1a-MO-injected kgg mutants and siblings. (E) β-cell
number increases further in Cdx1a-MO-injected kgg siblings and
mutants (mean±s.d.). (F) Midline convergence is further delayed
in Cdx1a-MO-injected kgg siblings and mutants. y-axis
indicates percentage of embryos in which β-cells have converged to the
midline. Note than in E and F, mutant and sibling data from B and C are
included for ease of comparison. Sample sizes for A-C were as follows. Wild
type (wt): 16hpf, n=38; 19hpf, n=36; 24hpf, n=60;
48hpf, n=17. Siblings (sib): 16hpf, n=38; 19hpf,
n=82 for A and C, n=45 for B; 24hpf, n=80 for A and
C, n=40 for B; 48hpf, n=33. Mutant (mut): 16hpf,
n=23; 19hpf, n=30; 24hpf, n=24; 48hpf,
n=23. Sample sizes for D-F were as follows. insulin data,
siblings+Cdx1a-MO (sib+MO): 19hpf, n=24; 24hpf, n=16.
Mutant+Cdx1a-MO (mut+MO): 19hpf, n=22; 24hpf, n=10. Sample
sizes for D were as follows. pdx1 data, wild type: 19hpf,
n=22; 24hpf, n=9. Siblings+Cdx1a-MO (sib+MO): 19hpf,
n=15; 24hpf, n=17. Mutant+Cdx1a-MO (mut+MO): 19hpf,
n=16; 24hpf, n=20.
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